Optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins

Marta Gallego, Leticia Mora, M-Concepción Aristoy and Fidel Toldrá

Meat PictureMarta Gallego and colleagues from Instituto de Agroquímica y Tecnología de Alimentos (CSIC) (ES) have developed a rapid, simple, and reliable method to quantify changes in protein abundance in meat. This method will help identify bioactive proteins with potential health benefits.

Proteomics is the study of the entire complement of proteins that is or can be expressed by a cell, tissue or organism.  The new approach developed in this study will be an indispensable tool for a rapid, precise and sensitive analysis of proteins in complex biological samples.

The method for label-free quantitative or qualitative analysis of proteins is useful because it is able to identify bioactive peptides more rapidly. In this study, proteins from pork were separated by one-dimensional gel electrophoresis following trypsin digestion, and quantified using nanoliquid chromatography coupled to a quadrupole/time-of-flight (Q/ToF) mass spectrometry. Relative quantitation was based on measurement of mass spectral peak intensities, which were correlated with protein abundances. The method was shown to be linear, robust and accurate.

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Highlights:

  1. The method advances the field of proteomics by providing a rapid, simple, and reliable approach to quantify changes in protein abundance in biologically complex samples
  2. The new method has good linearity, robustness and accuracy in identifying proteins